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Extraction, purification, structural character
The recovery and purity of the two flavonoids were over 95% and 90% respectively . The results showed that the purity of vitexin from artemisia annua leaves was 99% and the recovery was 96.2% with a two-phase solvent system consisting of n-hexane, ethyl acetate, methanol and water at the volume ratio of 7∶10∶7∶10 . 3.6.
Gas chromatography (GC) conditions: HP-5MS (30 mx0.25 mmx0.25 μm, Agilent, 6890 N-5975B) chromatographic column was used, the injector temperature was 230 ℃, the carrier gas was high purity He, the flow rate was 1.2 mL / min, the detector temperature was 240 °C, and the split ratio was 60:1.
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